SOLID PHASE SEQUENCE-INDEPENDENT NUCLEIC ACID ASSEMBLY
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May 9, 2016
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May 7, 2015
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Disclosed herein are methods of assembling nucleic acid fragments, such as non overlapping nucleic acid fragments, in an orientation-specific, sequence-independent way. The methods entail annealing one or more nucleic acid fragments to be assembled onto a solid phase via matching barcodes having sequences complementary to the solid phase oligos, ligating the nucleic acid fragments by end-to-end ligation, and recovering the ligated nucleic acid from the solid phase.
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