NON-TRANSGENIC HAPLOID INDUCER LINES IN CUCURBITS
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United States of America Patent
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Nov 8, 2018
app pub date -
Nov 8, 2016
filing date -
Nov 8, 2016
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Abstract
The present invention relates to a mutant plant of the Cucurbitaceae family comprising a modified CENP-C gene, which mutant plant when crossed to a wild-type plant having 2n chromosomes produces progeny, at least 0.1% of which have n chromosomes. The modification comprises for example a mutation in the CENP-C gene that leads to the occurrence of a premature stop codon or to a non-conservative amino acid change in the C-terminal region of the encoded protein. The plant is for example a Cucumis sativus plant and the modified CENP-C gene encodes a modified CENP-C protein that comprises at least one not-tolerated amino acid change or a premature stop codon in the C-terminal region, when compared to the CENP-C protein of SEQ ID No:1, in particular a mutation selected from E674K and S650N. The plant may also be a Cucumis melo plant wherein the modified CENP-C gene encodes a modified CENP-C protein that comprises at least one not-tolerated amino acid change or a premature stop codon in the C-terminal region, when compared to the CENP-C protein of SEQ ID No:2.
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