METHOD FOR GENERATING HIGHER ORDER GENOME EDITING LIBRARIES

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United States of America Patent

APP PUB NO 20190330616A1
SERIAL NO

16472645

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention pertains to a novel method for the generation of highly diverse RNA expressing vectors and vector libraries for use in targeted gene knock out, knock down and genome modification approaches. The invention pertains to a method for generating such higher order libraries without the need of classical cloning technologies. This is particularly useful for libraries based on large vectors wherein a sequence cannot be easily mutated with classical mutagenesis methods. The vectors and libraries generated according to the methods of the invention are in particular for RNA assisted silencing technologies such as RNA interference, and for targeted genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system or similar RNA/DNA-encoded gene perturbation systems which use small guide RNAs to target the CRISPR complex to a specific genomic sequence. The invention provides also kits comprising the materials for performing the methods of the invention.

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Patent Owner(s)

Patent OwnerAddress
JOHANN WOLFGANG GOETHE-UNIVERSITÄTSENCKENBERGANLAGE 31 FRANKFURT AM MAIN 60325

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
DE, BRUYN Rahel Wuerzburg, DE 1 0
DIEHL, Valentina Frankfurt am Main, DE 1 0
ERNST, Andreas Frankfurt am Main, DE 25 196
KAULICH, Manuel Frankfurt am Main, DE 1 0
WEGNER, Martin Bad Nauheim, DE 9 43
WIECHMANN, Svenja Frankfurt am Main, DE 1 0

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