HIGH-THROUGHPUT MICROFLUIDIC-BASED METHODS FOR RECORDING DEFECATION MOTOR PROGRAM (DMP) EVENTS IN NEMATODES
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United States of America
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Issued Date -
Jan 16, 2020
app pub date -
Jun 25, 2019
filing date -
Jun 25, 2019
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Abstract
The present disclosure provides methods and systems for performing, observing, and/or recording defecation motor program (DMP) events using microfluidic devices. The methods may be performed wherein the nematodes ingest fluorescent or color material and are then loaded in a microfluidic chip and stimulated to feed and defecate. DMP events are observed with use of a fluorescent microscope. In other methods, a microfluidic device with two or more electrodes is used to record electrical events of the DMP.
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