CONSTRUCTS FOR CONTINUOUS MONITORING OF LIVE CELLS
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United States of America
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Jan 16, 2020
app pub date -
Sep 21, 2017
filing date -
Sep 21, 2017
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Abstract
The present invention provides for methods to obtain multiple information-rich samples at different time points from the same cell while minimally disrupting the cell. The subject matter disclosed herein is generally related to nucleic acid constructs for continuous monitoring of live cells. Specifically, the subject matter disclosed herein is directed to nucleic acid constructs that encode a fusion protein and a construct RNA sequence that induce live cells to self-report cellular contents while maintaining cell viability. The present invention may be used to monitor gene expression in single cells while maintaining cell viability.
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