MULTI-COLOR IMAGING USING SALVAGED FLUORESCENCE
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United States of America
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Feb 27, 2020
app pub date -
Aug 26, 2019
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Aug 26, 2019
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Abstract
Methods and systems for fluorescence imaging are described herein. The method can include: receiving a fluorescence signal including an excitation signal, a first emission signal for a first fluorophore having a first emission spectra, and a second emission signal for a second fluorophore having a second emission spectra; filtering the fluorescence signal to: isolate a first channel encompassing at least one of: a bandwidth of at least 1 nm within which an emission intensity of the first emission spectra is at least twice an emission intensity of the second emission spectra, a bandwidth having a rising edge of the first emission spectra; and at least 10% by height of a rising edge of the first emission spectra, and produce a channel including the fluorescence signal less the first channel; and directing the first channel and the resulting channel to different regions of one or more cameras for collecting fluorescence emissions.
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