PROCESS FOR THE PURIFICATION OF LACTO-N-NEOTETRAOSE
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United States of America Patent
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Aug 11, 2022
app pub date -
Jun 10, 2020
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Jun 14, 2019
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Abstract
Disclosed is a process for the purification of LNnT (lacto-N-neotetraose) from a fermentation broth, the process comprises subjecting a fermentation broth to a first step of membrane filtration, thereby providing a filtrated solution, such filtrated solution is subjecting to a second step of simulated moving bed chromatography, obtaining a purified solution thereof, then subjecting this purified solution to a third step of crystallization, obtaining crystals containing the LNnT of interest, and subjecting the crystals to a fourth and final step of drying, thereby providing a highly purified powder of LNnT.
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