Method for separation and purification of n-acetylglucosamine

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United States of America Patent

PATENT NO 11555049
APP PUB NO 20220324896A1
SERIAL NO

17844994

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Abstract

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The disclosure relates to a method for separation and purification of N-acetyl-glucosamine, and belongs to the technical field of biological engineering. In the disclosure, a raw material solution containing N-acetyl-glucosamine is obtained by microbial fermentation or by hydrolyzing the chitin. The raw material solution is subjected to flocculation pretreatment, and continuous centrifugation or pressure filtration is performed to remove suspended solids such as microorganisms, proteins and polysaccharides to obtain clear liquid. Double-stage ion exchange chromatography is performed to remove impurities such as charged organic molecules and inorganic salts. Membrane concentration is performed to efficiently remove water to improve the concentration of the target product. Spray drying or further evaporation concentration and crystallization are performed. Finally drying is performed to obtain an N-acetyl-glucosamine crystal of which the purity is more than 99%.

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Patent Owner(s)

Patent OwnerAddress
JIANGSU HARVERS BIOTECH CO LTDNO 888 GANGNAN ROAD JIANGSU ZHENJIANG

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Ding, Chunhua Zhenjiang, CN 2 0
Zhang, Wenjie Zhenjiang, CN 54 106

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