GLYCOENGINEERING USING LEISHMANIA CELLS
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United States of America Patent
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Sep 14, 2023
app pub date -
Jan 7, 2021
filing date -
Jan 7, 2020
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Abstract
The present application relates to Leishmania cells genetically engineered such that the formation of an O-linked GlcNAc on a polypeptide in the Leishmania cell is reduced or eliminated. The formation of the O-linked GlcNAc may be catalyzed in the Leishmania cell prior to said genetic engineering by at least one N-acetylglucosamine (GlcNAc)-transferase. Also provided herein are methods of making a polypeptide using a Leishmania cell described herein and polypeptides produced by the methods provided herein.
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