Method for Determining Conditions for Cultivation, and Method for Producing Desired Protein or Desired Peptide
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United States of America Patent
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Issued Date -
Feb 22, 2024
app pub date -
Jan 11, 2022
filing date -
Jan 15, 2021
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Abstract
Disclosed is a method for determining conditions for cultivating a plant body expressing a protein of interest or a peptide of interest in a 24-hour cycle in which a dark period and a light period are repeated alternately, including: a step of determining an optimum leaf temperature based on a capability to express a protein of interest or a peptide of interest; and a step of setting light and dark period leaf temperatures and light and dark period time lengths after a plant body is infected with a bacterium or a virus having a polynucleotide encoding the protein of interest or the peptide of interest so as to satisfy the following formula, the following formula being: Dark period leaf temperature (° C.)×Dark period time length (hour)+Light period leaf temperature (° C.)×Light period time length (hour)=Optimum leaf temperature (° C.)×24 (hour)±24.
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