Importance

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Abstract

A one-pot method of multiple protein labeling that is suitable for protein footprinting is disclosed, along with systems, auxiliary devices, kits, and non-transitory computer readable mediums relating to the same. Hydroxyl radicals are maintained at a concentration for a length of time sufficient to both react with trifluoromethyl precursors and a protein of interest, where the reaction with the trifluoromethyl precursors further maintains a trifluoromethyl concentration for a length of time sufficient to react with the protein of interest. The multiplex labeling achieves significant improvements over the state of the art labeling methods, both by increasing the types of amino acids which can be labeled in a single experiment and by increasing the overall labeling coverage.

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First Claim