METHOD FOR PREPARING PROTEIN NANOPARTICLE MICROSPHERES
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United States of America Patent
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Issued Date -
Apr 4, 2024
app pub date -
Dec 6, 2022
filing date -
Sep 30, 2022
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Abstract
A method for preparing protein nanoparticle microspheres includes: dispersing a protein powder in water, adjusting a pH of a solution of the protein powder to between 10.0 and 12.0, stirring, centrifuging, and collecting a first upper dispersion; adjusting the first upper dispersion to be neutral using a cation exchange resin, centrifuging, to yield a protein nanoparticle dispersion; adding eugenol to the protein nanoparticle dispersion, stirring, centrifuging, and removing a precipitant, to yield a core-shell nanoprotein dispersion comprising a eugenol core and a protein shell; and adding the core-shell nanoprotein dispersion to ultrapure water, dialyzing, centrifuging, collecting a second upper dispersion, freeze-drying the second upper dispersion, to yield protein nanoparticle microspheres.
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