EXPRESSION AND ENHANCED SECRETION OF ACTIVE MORINGA OLEIFERA COAGULANT PROTEIN IN BACILLUS
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United States of America Patent
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Jul 4, 2024
app pub date -
Sep 25, 2019
filing date -
Sep 25, 2019
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Abstract
A system and method for producing a recombinant Moringa oleifera coagulant protein (MO) in Bacillus includes the expression of a recombinant nucleic acid encoding the MO protein fused or linked to a Bacillus signal peptide (SP). The nucleic acid can also be modified by codon optimization for enhanced expression of the MO protein in bacteria. The recombinant MO protein has coagulation/flocculation activity and can be used in the treatment of contaminated water, particularly drinking water. Vectors and nucleic acid constructs encoding the MO protein and SP, and host bacterial cells are also disclosed.
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