SEPARATION OF HUMAN MILK OLIGOSACCHARIDES FROM A FERMENTATION BROTH
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United States of America Patent
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Issued Date -
Aug 29, 2024
app pub date -
Jun 14, 2022
filing date -
Jun 15, 2021
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Abstract
The invention relates to a method for recovery and purification of a neutral or sialylated human milk oligosaccharide (HMO) from a fermentation broth, comprising the steps of separating the fermentation broth to form a separated HMO-containing stream and a biomass waste stream, purifying the HMO-containing stream by nanofiltration, purifying the HMO-containing stream with an acidic cation exchange resin, concentrating the purified HMO-containing stream, and drying the purified HMO-containing stream to obtain a solidified neutral or sialylated HMO. Moreover, the invention also concerns a neutral or sialylated human milk oligosaccharide obtained by the inventive method, as well as its use in food, feed, and medical application.
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