WHOLE TRANSCRIPTOME ANALYSIS OF SINGLE CELLS USING RANDOM PRIMING
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United States of America Patent
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Issued Date -
Oct 3, 2024
app pub date -
Feb 16, 2024
filing date -
Nov 8, 2018
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Abstract
Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.
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