CRISPR-BASED PROTEIN BARCODING AND SURFACE ASSEMBLY
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United States of America Patent
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Oct 24, 2024
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Jul 11, 2022
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Jul 11, 2022
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Abstract
Biotechnological innovations have vastly improved the capacity to perform large-scale protein studies. The production and interrogation of custom protein libraries has proven important for a plethora of biological applications including multiplexed disease diagnostics, therapeutic antibody discovery, and directed evolution. The present invention relates to methods and compositions for use in making Cas-related fusion protein libraries barcoded with sgRNA sequences for applications in protein studies and for protein self-assembly on surfaces.
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