HIGH-THROUGHPUT SINGLE-CELL SEQUENCING WITH REDUCED AMPLIFICATION BIAS
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United States of America
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Issued Date -
Nov 14, 2024
app pub date -
May 3, 2024
filing date -
May 3, 2024
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Abstract
Provided herein are methods for preparing a sequencing library that includes nucleic acids from a plurality of single cells. In one embodiment, the methods include linear amplification of the nucleic acids. In one embodiment, the sequencing library includes whole genome nucleic acids from the plurality of single cells. In one embodiment, the nucleic acids include three index sequences. Also provided herein are compositions, such as compositions that include the nucleic acids having three index sequences.
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