SYNTHESIS OF TRINUCLEOTIDE AND TETRANUCLEOTIDE CAPS FOR MRNA PRODUCTION
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United States of America
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Nov 21, 2024
app pub date -
Mar 30, 2022
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Mar 30, 2022
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Abstract
Provided herein are methods of making trinucleotides and tetranucleotides for use as 5′ mRNA caps. The methods utilize a novel “top-down” strategy and provide for synthesis of oligonucleotides with higher yields and increased efficiency compared to traditional methods. A key step of the methods disclosed, herein can also be adapted to utilize a “one-pot” approach, resulting in an increase in the yield of the final oligonucleotide product.
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