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Abstract

A method for simultaneously separating and purifying myricetin-3-O-(2″-O-galloyl)-a-L-rhamnoside and myricetin-3-O-(4″-O-galloyl)-a-L-rhamnoside from Bayberry leaves, and the use of the compounds prepared by the method. The method includes: (1) alcohol extraction and concentration: mixing Bayberry leaves with an alcohol solution; (2) solid-phase extraction column adsorption: injecting the crude Bayberry leaf flavonol alcohol extract into a solid-phase extraction column; washing with a mobile phase for the first gradient elution; (3) preparative liquid chromatography purification: using a solid-phase chromatography column; wherein the solid-phase extraction powder obtained from step (2) is subjected to a second gradient elution with a mobile phase and then processed to obtain the target products: myricetin-3-O-(2″-galloyl)-α-L-rhamnoside monomer and myricetin-3-O-(4″-galloyl)-α-L-rhamnoside monomer.

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