IMPROVED CIRCULAR RNA FOR EXPRESSING THERAPEUTIC PROTEINS
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United States of America Patent
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Issued Date -
Jan 23, 2025
app pub date -
Oct 31, 2022
filing date -
Oct 31, 2022
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Abstract
The present invention is inter alia directed to improved circular RNA constructs comprising (i) at least one translation initiation sequence, (ii) at least one coding sequence (cds), (iii) at least one UTR sequence, and (iv) at least one poly(A) sequence. Further provided is linear precursor RNA for making such an improved circular RNA. The invention also relates to improved methods for preparing circular RNA and to improved methods of purifying circular RNA. Moreover, the invention relates to pharmaceutical compositions, vaccines, combinations, and kit or kit of parts comprising the circular RNA of the invention. Also provided are methods of treating or preventing disorders or diseases, and first, second, and further medical uses.
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