METHOD FOR PRODUCING HAPLOID, AND DOUBLED HAPLOID BETA VULGARIS PLANTS BY MICROSPORE CULTURE
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United States of America
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Issued Date -
Feb 6, 2025
app pub date -
Dec 22, 2022
filing date -
Dec 22, 2022
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Abstract
The present invention relates to a method for the production of haploid, polyhaploid and/or doubled haploid embryos, calli, seeds and/or plants of the species Beta vulgaris from isolated microspore cultures, more specifically to a method comprising contacting the isolated microspores with a histone deacetylase inhibitor (HDACi) and a complex protein composition. The present invention also provides a kit for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris from at least one isolated microspore as well as the use of a histone deacetylase inhibitor (HDACi) and a complex protein composition for producing a haploid, polyhaploid and/or doubled haploid embryo, callus, seed and/or plant of the species Beta vulgaris. Finally, the present invention also relates to a population of haploid, polyhaploid and/or doubled haploid Beta vulgaris plants directly derived from a single flower, single inflorescence or single bud.
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