BACILLUS EXPRESSION SYSTEM

Number of patents in Portfolio can not be more than 2000

United States of America

APP PUB NO 20250043293A1
SERIAL NO

17311675

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Abstract

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An inducible promoter expression system based on the T7 RNA Polymerase (T7 RNAP), lactose repressor (Lad), and a chimeric T7lac promoter (PT7lac), which can be integrated as a single copy into the B. subtilis genome. In the absence of IPTG. Lad strongly represses T7RNAP and PT7lac, and expression of an exemplary ORF—here superfolder green fluorescent protein (sfGFP) reporter protein—is undetectable by flow cytometry. Addition of IPTG de-represses PT7lac, and simultaneously induces expression of T7RNAP, resulting in very high sfGFP levels.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
CASTILLO-HAIR, Sebastian M Houston, US 1 0
TABOR, Jeffrey J Houston, US 4 0

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