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Abstract

The present disclosure provides a purification method of a Mycobacterium-derived nanocarrier protein, and belongs to the technical field of protein purification. In the present disclosure, the purification method includes the following steps: providing an Escherichia coli total protein solution, where the Escherichia coli total protein solution includes a Mycobacterium-derived nanocarrier protein, and the Mycobacterium-derived nanocarrier protein is fused with a 1×Flag tag at a C-terminus of a subunit; subjecting the Escherichia coli total protein solution to Flag tag affinity chromatography purification to obtain a crude extract of the Mycobacterium-derived nanocarrier protein; and subjecting the crude extract of the Mycobacterium-derived nanocarrier protein to gel exclusion chromatography purification to obtain a pure product of the Mycobacterium-derived nanocarrier protein. The purification method has simple and easy operations.

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First Claim