METHODS FOR CHARACTERIZING A PROTEIN OF INTEREST
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United States of America
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Mar 6, 2025
app pub date -
Aug 28, 2024
filing date -
Aug 28, 2024
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Abstract
Methods can be conducted to prevent disulfide scrambling in liquid chromatography-mass spectrometry analysis of a protein of interest. In particular, methods can be conducted by employing a NEM analog, such as maleimide, and a heavy isotope counterpart of the NEM analog, such as a heavy isotope substituted maleimide, to alkylate a protein of interest and to alkylate a peptide digest of the protein of interest under conditions that prevent disulfide scrambling and subjecting the samples to a liquid chromatography-mass spectrometry analysis.
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