ARTIFICIAL EXONIC BARCODE SYSTEM
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United States of America
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Issued Date -
Mar 20, 2025
app pub date -
Aug 19, 2024
filing date -
Aug 19, 2024
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Abstract
The present disclosure is generally directed to an artificial exonic barcode system. The exonic barcodes comprise a nucleotide sequence comprising from 5′ to 3′ a 5′ barcode, an intron, and a 3′ barcode, and the disclosure is further directed to a library of these exonic barcodes. The disclosure also describes a method of generating the exonic barcode library and using the library of exonic barcodes in a method of screening for efficiency of transformation and/or expression of one or more genetic constructs in a subject. Primers and probes were also designed for validation of these exonic barcodes and corresponding methods.
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