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Abstract

A digital immunoassay detection and analysis method for precise quantification of proteins are provided. The digital immunoassay detection and analysis method for precise quantification of proteins includes the steps of: (s1) providing an aqueous phase mixture; (s2) mixing the aqueous phase mixture with a chromogenic substrate and an oil phase to form a first mixture; (s3) shearing of the first mixture to form a sheared water in oil second mixture; and (s4) measuring the first micro droplet to obtain the number of first droplets and obtain the quantitative detection result of protein. The second mixture contains a first micro droplet and a second micro droplet, The first micro droplet is a micro droplet containing a positive signal complex. The second micro droplet is a micro droplet containing a negative signal complex.

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