METHOD OF YEAST PROPAGATION IN A LOW-COST, TWO-PHASE PROCESS STREAM
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United States of America Patent
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Issued Date -
Mar 27, 2025
app pub date -
Jul 17, 2024
filing date -
Jul 17, 2024
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Abstract
The present invention relates to a method of propagating genetically modified yeasts or even any other yeasts that suffer a positive Crabtree effect or any other microorganisms that use sugars for growth (glucose or equivalent glucose), wherein the feeding medium inhibits the optimal exponential growth predicted throughout the process, such a method capable of providing greater process economicity (CAPEX and OPEX), increased productivity and yield (Yx/s) and reduced CAPEX due to greater cell expansion in a single stage of reactor. Particularly, the present invention describes a process for producing second generation ethanol by using different combinations of low-cost streams existing in an integrated 2G or 1G/2G ethanol production plant, combined with the unique batch propagation strategy fed in at least two exponential feeding phases, in just one cycle, with the propagation step occurring in a single reactor.
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