RECOMBINANT TYPE I ALLERGEN OF ARTEMISIA ANNUA POLLEN, AND PREPARATION METHOD AND USE THEREOF
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United States of America
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Apr 3, 2025
app pub date -
Dec 30, 2022
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Dec 30, 2022
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Abstract
A recombinant type I allergen of Artemisia annua pollen (Art a 1), a coding gene, an expression method, and a purification method thereof are provided. By different combinations of codon optimization, different signal peptides, expression vectors and strains, the Art a 1 obtained reaches the expression level of more than 200 mg/L, with a purity of more than 99%, and the activity of the Art a 1 is equivalent to that of a native protein, and the Art a 1 can be used for the desensitization immunotherapy and diagnosis of Artemisia pollen allergy.
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