ALDITOL OXIDASE AND APPLICATION THEREOF
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United States of America
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Issued Date -
Apr 10, 2025
app pub date -
Nov 30, 2023
filing date -
Nov 30, 2023
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Abstract
An alditol oxidase and application thereof. The method uses D-glucose as a substrate to dock the alditol oxidase derived from Streptomyces coelicolor A3, and selects amino acid residues around an active center for saturation mutagenesis, and screens for alditol oxidase with D-glucose oxidizing activity by plate color development. An amino acid sequence of the alditol oxidase is shown in SEQ ID NO: 2 or SEQ ID NO: 4. The alditol oxidase has the activity of converting D-glucose to D-gluconic acid and D-glyceraldehyde to D-glyceric acid. By using the alditol oxidase, the conversion of D-glucose to pyruvic acid is realized by only three enzymes for the first time and does not depend on any coenzyme.
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