Process for the purification of carbohydrate containing enzymes
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United States of America Patent
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Mar 18, 1980
Grant Date -
N/A
app pub date -
Jul 31, 1978
filing date -
Jul 31, 1978
priority date (Note) -
Expired
status (Latency Note)
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Abstract
This invention relates to a method for purifying a carbohydrate containing enzyme which is a desired enzyme preferred to be separated from a mixture. The method comprises the steps of mixing a solution containing the carbohydrate containing enzyme with a carbohydrate modifying reagent. The carbohydrate modifying reagent reacts with the carbohydrate attached to the enzyme, thereby modifying its chemical structure. The modified enzyme is then separated from the other undesirable enzymes or proteins in the mixture by a suitable chemical separation method, for example, gel filtration chromatography. The method was used in the separation of glucose oxidase from catalase, a separation which by previous methods was very inefficient.
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