Method of magnetic separation of cells and the like, and microspheres for use therein
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United States of America Patent
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Oct 28, 1980
Grant Date -
N/A
app pub date -
Feb 28, 1979
filing date -
Feb 28, 1979
priority date (Note) -
Expired
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Abstract
Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.
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