Determination of serine proteases
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United States of America Patent
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Jun 30, 1981
Issued Date -
N/A
app pub date -
Oct 22, 1979
filing date -
Dec 1, 1976
priority date (Note) -
Expired
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Abstract
Serine proteases in a substance such as blood are determined by contacting the substance with a novel chromogenic or fluorgenic substrate for serine proteinases and spectrophotometrically measuring the quantity of a chromophore or fluorescent compound released from the substrate by serine proteases in the substance. The novel substrate has the amino acid sequence-ILe-A-Gly-Arg-wherein A is Asp or Glu substituted in the carboxylic group by esterification or amidation. The novel substrate increases sensitivity and accuracy of the determination.
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