Method for determining the concentration of an L-amino acid in fermentation
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United States of America Patent
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Nov 10, 1981
Grant Date -
N/A
app pub date -
Mar 27, 1979
filing date -
Mar 28, 1978
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an oxygen-sensitive electrode or a carbon dioxide gas-sensitive electrode combined with fixed microbial cells of a micro-organism capable of assimilating said carbon source or decarboxylating said L-amino acid with a sample solution and sensing electrically the rate of current decrease which is caused by the consumed oxygen in the solution in proportion to the concentration of carbon source; or the electric motive force which is caused by the liberation of carbon dioxide in the sample solution in proportion to the concentration of L-amino acid. Microbial electrodes and systems useful in carrying out the aforementioned method.
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