Means of preparation and applications of liposomes containing high concentrations of entrapped ionic species
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United States of America Patent
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Jan 12, 1982
Issued Date -
N/A
app pub date -
Feb 20, 1980
filing date -
Feb 22, 1979
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Abstract
Unilamellar vesicles comprising a lipid bilayer, an ionophore being incorporated in said lipid bilayer, a chelating agent entrapped within the vesicles, and an effective amount of physiologically compatible cation bound to said chelating agent within the vesicles. Unilamellar vesicles are loaded by incubating the vesicles with the cation, terminating the incubation and recovering the loaded vesicles by chromatography. In some applications of the invention, the cation may be a radioactive tracer, in which case the vesicles can be administered to the human host and observed by scintillation techniques to produce a radioimage which is useful for diagnostic purposes.
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