Nucleosidic phosphorylating agent and methods
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United States of America Patent
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Jan 12, 1982
Grant Date -
N/A
app pub date -
Dec 26, 1979
filing date -
Dec 26, 1979
priority date (Note) -
Expired
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Abstract
Compounds of the formula ##STR1## where R is a base labile substituted-phenyloxy protecting group, are useful as phosphorylating reagents in the synthesis of oligonucleotides by a phosphotriester method. Such compounds are made by the reaction of .beta.-cyanoethanol and a corresponding R-phosphorodichloridate. A method of phosphorylating involves reacting such compounds, in the presence of base, with a nucleoside or a polynucleotide having a free 3' hydroxyl group, having the 5'--0 position protected by an organic group labile in acidic medium and having any phosphotriester linking groups protected by an organic group labile in strong basic medium.
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