Protein specific fluorescent microspheres for labelling a protein
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United States of America Patent
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Apr 20, 1982
Issued Date -
N/A
app pub date -
Apr 25, 1980
filing date -
Aug 27, 1976
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Expired
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Abstract
Highly fluorescent, stable and biocompatible microspheres are obtained by copolymerizing an acrylic monomer containing a covalent bonding group such as hydroxyl, amine or carboxyl, for example, hydroxyethylmethacrylate, with an addition polymerizable fluorescent comonomer such as dansyl allyl amine. A lectin or antibody is bound to the covalent site to provide cell specificity. When the microspheres are added to a cell suspension the marked microspheres will specifically label a cell membrane by binding to a specific receptor site thereon. The labeled membrane can then be detected by fluorescence of the fluorescent monomer.
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