Process for recovering Cu,Zn-superoxide dismutase from yeast
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United States of America Patent
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Jul 20, 1982
Grant Date -
N/A
app pub date -
May 13, 1980
filing date -
May 17, 1979
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Cu,Zn-superoxide dismutase is recovered from yeast by plasmolysis with a small amount of ether or any other water immiscible, organic solvent and subsequent autolysis in water at a temperature of 25.degree. to 50.degree. C. and pH 5 to 9, following which the precipitate is removed and the superoxide dismutase is purified and isolated from the residual liquid, in particular by chromatography on carboxymethyl cellulose at pH 4.7 to 5.5. Cu,Zn-superoxide dismutase from Saccharomyces cerevisiae has the amino acid sequence: ##STR1## where Cys-57 and Cys-146 form a disulfide bond.
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