Fluorescent antibody composition for immunofluorometric assay
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United States of America Patent
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Jul 27, 1982
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N/A
app pub date -
Nov 26, 1975
filing date -
Nov 26, 1975
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Expired
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Abstract
A new family of fluorescent antibodies (FAB) and a method for their synthesis is disclosed for use in an apparatus and method wherein the effects of background fluorescence are virtually eliminated from fluorescence measurements. The novel fluorescent antibodies consist of antibodies conjugated with rare-earth chelates which have unique fluorescent properties. These fluorescent antibodies are useful because when excited with short pulses of light, they exhibit fluorescent decay times which are much longer than typical background fluorescence at room temperature and with a spectral width far narrower than typical background fluorescence.
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