Process and apparatus for the staining of biological samples in which fluids of differing specific densities are successively introduced to the samples comprises a closed chamber to receive the samples for treatment, the chamber being provided with flow passages at the top and bottom and is in communication with an under pressure generating device so that fluids may be introduced into the chamber either from the top or the bottom depending upon whether a succeeding fluid is of lesser, or greater, specific density than the preceding fluid.
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