Extraction, separation and recovery of diterpene glycosides from Stevia rebaudiana plants
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United States of America Patent
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Nov 30, 1982
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N/A
app pub date -
May 21, 1981
filing date -
May 21, 1981
priority date (Note) -
Expired
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Abstract
A process for recovering diterpene glycosides from the Stevia rebaudiana plant includes the steps of sequentially extracting plant material with a first solvent of intermediate polarity to extract plant substances which tend to interfere with a liquid chromatographic separation of the glycosides, and then with a second solvent of high polarity to extract glycosides, and chromatographically separating the extracted glycosides by introducing them onto a liquid chromatography column having a packing of an oxygen-containing organic stationary phase covalently bonded through a silicon atom to an inorganic support, eluting them with a solvent of polarity higher than that of the first solvent but lower than that of the second solvent, and collecting individually eluate fractions rich in respective glycosides.
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