Apparatus for high pressure peptide synthesis
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United States of America Patent
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Dec 7, 1982
Grant Date -
N/A
app pub date -
Mar 10, 1981
filing date -
Mar 10, 1981
priority date (Note) -
Expired
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Abstract
An apparatus for synthesizing a polypeptide chain on an insoluble solid support in a continuous flow reactor by performing a series of stepwise coupling reactions under high pressure is disclosed. A deprotecting agent reservoir, a solvent reservoir, a neutralizing solution reservoir, and a plurality of amino acid reservoirs are provided. Each reservoir can be selectively coupled to the reactor and a source of high pressure inert gas or an appropriate pump can be used to force the fluids as needed to the reactor. The reactor also includes a recycle loop whereby the amino acid from a particular reservoir can be recycled through the reactor under high pressure.
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