Importance

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Abstract

A system for the detection of a biological analyte of interest is disclosed which comprises a microencapsulated fluorescer material which has been conjugated to an immunological specie specific to the biological analyte of interest, a means of disrupting the capsule containing the fluorescer and an energy source other than electro-magnetic radiation which is capable of activating the fluorescer. A method for the qualitative and/or quantitative detection of a biological of interest is disclosed, which comprises: (a) labeling an immunological specie specific to the analyte of interest with a microencapsulated fluorescer material which is biologically compatible with such specie; (b) contacting the microencapsulated fluorescer labeled specie and the biological of interest to form a microencapsulated fluorescer labeled specie/biological complex; (c) separating the microencapsulated fluorescer labeled specie/biological complex; (d) distrupting the capsule containing the fluorescer label thus freeing it to solution; (e) contacting the freed fluorescer with an energy source other than electro-magnetic radiation which is capable of activating the fluorescer label; and (f) determining the presence of and/or measuring the quantity of chemiluminescent light emitted.

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First Claim