Process for preparing artificial red cells
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United States of America Patent
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Mar 8, 1983
Grant Date -
N/A
app pub date -
Apr 11, 1980
filing date -
Apr 11, 1980
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A process for preparing artificial red cells comprising microdroplets of aqueous, stroma-free hemoglobin solution encapsulated in membranes of polymerized hemoglobin, said process comprising forming microdroplets of hemoglobin solution in a continuous oil phase, cross-linking the hemoglobin at the surface of the microdroplets with a suitable cross-linking agent to form the artificial red cells and recovering the cells. In one embodiment, a liquid membrane encapsulation technique employing a water-soluble cross-linking agent is used to form the cells. In another embodiment an oil-soluble cross-linking agent is present in the oil phase containing the hemoglobin microdroplets.
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