Process for isolating Cu, Zn-superoxide dismutase from aqueous solutions containing said enzyme together with accompanying proteins
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United States of America Patent
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Jun 28, 1983
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N/A
app pub date -
Nov 10, 1981
filing date -
May 17, 1979
priority date (Note) -
Expired
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Abstract
Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.0 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups. The process lends itself to use on an industrial scale and provides a high yield of pure SOD.
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