Method for quantitatively determining a particular substrate catalyzed by a multisubstrate enzyme
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United States of America Patent
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Sep 13, 1983
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N/A
app pub date -
May 13, 1982
filing date -
Aug 25, 1980
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Abstract
A method for control of relative enzyme activity for analytical purposes. The activity of a multisubstrate enzyme may be controlled as a function of the electrical potential applied to the enzyme. The enzyme is preferrably incorporated into a thin layer electrochemical cell laminate having exterior membrane layers and an interior enzyme layer. A control electrode located within the enzyme layer applies an electrical potential to the enzyme. An intermediate electron transfer agent may be used to transfer electrons to and from the enzyme and the control electrode.
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