Process for the preparation of antibodies
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United States of America Patent
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Dec 27, 1983
Issued Date -
N/A
app pub date -
Apr 28, 1982
filing date -
Oct 16, 1980
priority date (Note) -
Expired
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Abstract
A novel peptide of the formula H--Ser--Arg--Arg--Ala--Gln--Asp--Phe--Val--Gln--Trp--Leu--Met--Asn--Thr--OH is the 16-29 fragment of glucagon and can be conjugated with a protein such as albumin or modified albumin using glutaraldehyde or 3-(2'-benzothiazolyl-dithio) propionate succinimide ester as a conjugation reagent. The conjugated peptide can be used to sensitize a mammal by administering the conjugated peptide subcutaneously, after which the blood is collected from the mammal and antibodies concentrated from the blood, which antibodies are then useful in assay methods for trace amounts of glucagon in human blood.
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