Standard or control material for glysocylated or total hemoglobin determination
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United States of America Patent
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Aug 14, 1984
Grant Date -
N/A
app pub date -
Jul 16, 1981
filing date -
Jul 16, 1981
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A control material for use in the determination of glycosylated and total hemoglobin, and a method of preparing the same, are disclosed. The control is prepared by the steps of hemolyzing red blood cells, preferably in the presence of a detergent, followed by removal of stroma and lipids from the hemoglobin with an organic solvent such as toluene in the presence of a polyhydroxy compound such as sucrose. A hemoglobin-rich fraction is separated from the resulting mixture and dialyzed against a solution of a polyhydroxy compound. The dialysate may be lyophilized to provide a stable, dry product suitable for reconstitution.
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