Method of obtaining antibodies
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United States of America Patent
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Oct 23, 1984
Grant Date -
N/A
app pub date -
Jan 25, 1982
filing date -
Jan 25, 1982
priority date (Note) -
Expired
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Abstract
Antibodies specific to glycosylated hemoglobin and substantially free of cross-reactivity with nonglycosylated forms of hemoglobin are obtained by administering a synthetic antigen to a host animal. The antigen comprises a glycosylated synthetic peptide having an amino acid sequence corresponding to that of the NH.sub.2 -terminus of the .beta.-chain of hemoglobin. The peptide comprises a sequence of between about four and ten, and preferably seven, amino acids, and is conjugated with glucose and a protein. The protein preferably comprises an immunoglobulin which is foreign to the host animal.
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