Sensitive and rapid diagnosis of viroid diseases and viruses
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United States of America Patent
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Oct 30, 1984
Grant Date -
N/A
app pub date -
Dec 3, 1981
filing date -
Dec 3, 1981
priority date (Note) -
Expired
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Abstract
A rapid and sensitive method for diagnosing plant viroid diseases and viruses. Plant sap is bound to a solid support and the bound sample probed with a radioactively labelled DNA that is complementary to the viroid or to the nucleic acid of the virus being diagnosed. The radioactively labelled cDNA hybridizes with that viroid or virus RNA or DNA for which it is specific. DNA-RNA and DNA-DNA hybrids are detected by autoradiographic examination of the hybridized material.
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