Method and apparatus for separating lymphocytes from anticoagulated blood
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United States of America Patent
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Dec 11, 1984
Grant Date -
N/A
app pub date -
Feb 18, 1983
filing date -
Feb 18, 1983
priority date (Note) -
Expired
status (Latency Note)
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Abstract
The present invention relates to apparatus and method for separating, by centrifugation, blood lymphocytes from other leukocytes in a blood sample. Small dense particles, either wetted or suspended in a physiological, hydrophilic medium, and a chemically inert hydrophobic barrier material are initially located in a suitable container. When the blood sample is added to the container, the particles are phagocytized by the monocytes and granulocytes, so as to increase their specific gravities. Upon centrifugation, the barrier material, whose specific gravity is only slightly greater than the specific gravity of the lymphocytes and less than the respective specific gravities of the phagocytic cells which have ingested particles and of the erythrocytes (red blood cells) in the blood sample, forms a rigid barrier therebetween, allowing harvesting of the lymphocytes, substantially free of contamination.
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